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<t>(A)</t> <t>C57BL/6</t> male mice were orthotopically implanted with 20,000 SB28 and treated with 4 g/L sitagliptin in water bottle starting on Day 0. Mice were intraperitoneally injected with 200 μg anti-CD8 or IgG2b isotype control antibodies on Days -1 and 0 and then with 100 μg every 5 days post intracranial injection until humane endpoint. (B) Kaplan-Meier curve of SB28-bearing mice treated vehicle/sitagliptin + anti-CD8/IgG2b. n=10/group combined from 2-independent experiments. (C) SB28 were treated with sitagliptin at 100 μM for two days and live population frequency evaluated by flow cytometry. n=3; ns=not significant by t-test.
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(A) C57BL/6 male mice were orthotopically implanted with 20,000 SB28 and treated with 4 g/L sitagliptin in water bottle starting on Day 0. Mice were intraperitoneally injected with 200 μg anti-CD8 or IgG2b isotype control antibodies on Days -1 and 0 and then with 100 μg every 5 days post intracranial injection until humane endpoint. (B) Kaplan-Meier curve of SB28-bearing mice treated vehicle/sitagliptin + anti-CD8/IgG2b. n=10/group combined from 2-independent experiments. (C) SB28 were treated with sitagliptin at 100 μM for two days and live population frequency evaluated by flow cytometry. n=3; ns=not significant by t-test.

Journal: bioRxiv

Article Title: Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activity

doi: 10.64898/2026.03.31.715681

Figure Lengend Snippet: (A) C57BL/6 male mice were orthotopically implanted with 20,000 SB28 and treated with 4 g/L sitagliptin in water bottle starting on Day 0. Mice were intraperitoneally injected with 200 μg anti-CD8 or IgG2b isotype control antibodies on Days -1 and 0 and then with 100 μg every 5 days post intracranial injection until humane endpoint. (B) Kaplan-Meier curve of SB28-bearing mice treated vehicle/sitagliptin + anti-CD8/IgG2b. n=10/group combined from 2-independent experiments. (C) SB28 were treated with sitagliptin at 100 μM for two days and live population frequency evaluated by flow cytometry. n=3; ns=not significant by t-test.

Article Snippet: Albino C57BL/6 mice (B6(Cg)- Tyr c-2J /J, 000058, The Jackson Laboratory) were purchased at 5 weeks of age.

Techniques: Injection, Control, Flow Cytometry